Acid phospholipase A in lysosomes of the bovine adrenal medulla.

نویسندگان

  • H Blaschko
  • A D Smith
  • H Winkler
  • H van den Bosch
  • L L van Deenen
چکیده

Kogut, M., Pollock, M. R. & Tridgell, E. J. (1956). Biochem. J. 62,391. Lowry, 0. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). J. biol. Chem. 193,265. Manson, E. E. D., Pollock, M. R. & Tridgell, E. J. (1954). J. gen. Microbiol., 11, 493. Miller, G., Bach, G. & Markus, Z. (1965). Biotech. Bioengng, 7, 517. Pollock, M. R. (1952). Brit. J. exp. Path. 83, 587. Pollock, M. R. & Torriani, A. M. (1953). C.R. Acad. Sci., Pari, 237,276. Ron-Zenziper, E. & Citri,N. (1963). Nature, Lond., 198,887. Sabath, L. D. & Abraham, E. P. (1965). Antimicrobial Agents and Chemotherapy, p. 392. Sabath, L. D. & Abraham, E. P. (1966). Biochem. J. 98, lec. Sabath, L. D., Jago, M. & Abraham, E. P. (1965). Biochem. J. 96, 739.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Lysosomal phospholipases A1 and A2 of bovine adrenal medulla.

1. [(32)P]Lecithin and [(32)P]phosphatidylethanolamine were prepared by incubating rat liver mince with [(32)P]phosphate. With these (32)P-labelled phospholipids conditions for the quantitative assay of phospholipase A activity were established. 2. The distribution of phospholipase A activity between subcellular fractions of the bovine adrenal medulla was determined. Phospholipases A(1) and A(2...

متن کامل

Isolation of a protein from the plasma membrane of adrenal medulla which binds to secretory vesicles.

Solubilized proteins of the plasma membrane of bovine adrenal medulla were fractionated on the basis of their affinity for secretory vesicles. The isolation procedure included preparation of a highly purified fraction of plasma membranes, its solubilization in detergent, and application to a column prepared from glutaraldehyde-fixed chromaffin granules. Using this technique, one major polypepti...

متن کامل

Purification of aromatic L-amino acid decarboxylase from bovine brain with a monoclonal antibody.

Aromatic L-amino acid decarboxylase was purified from bovine brain for the first time by affinity chromatography using a monoclonal antibody to the enzyme, and it was compared with the decarboxylase purified from bovine adrenal medulla by the same procedure. The monoclonal antibody was produced from a hybridoma established for the enzyme highly purified from bovine adrenal medulla. The Mr value...

متن کامل

Glycoprotein II from adrenal chromaffin granules is also present in kidney lysosomes.

Glycoprotein II (GP II) is a protein found in the membranes of chromaffin granules from adrenal medulla. Immunoblotting (one- and two-dimensional) revealed that this antigen is also present in liver and in kidney. Subcellular fractionation of the latter organ indicated that GP II was present in lysosomes. This was confirmed by immunoelectron microscopy. The antiserum against GP II immunolabelle...

متن کامل

Secretion and Endocytosis in Insulin-stimulated Rat Adrenal Medulla Cells

Insulin was used to deplete the adrenalin stores of rat adrenal medulla cells. Release of secretion was observed to occur by exocytosis. In addition, during the stages of massive release of secretory granules, the insulin-treated preparations showed greatly enhanced endocytic uptake of horseradish peroxidase. The tracer was taken up within vesicles, tubules, multivesicular bodies, and dense bod...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • The Biochemical journal

دوره 103 3  شماره 

صفحات  -

تاریخ انتشار 1967